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Package service

 

Polyclonal Antibody Service
Package Number
Description
Package Contents
Time
Price
Polyclonal package 1
(From protein to antiserum)
l  Customer provides antigen protein
l  Protein will be checked by SDS-PAGE
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  1-2ml pre-bleed
l  40-60ml post-bleed
l  Project datasheet including ELISA data
10-12 weeks
$575.00
Polyclonal package 2 (From protein to antigen purified antibody)
l  Customer provides antigen protein
l  Protein will be checked by SDS-PAGE
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Dot blot confirmation
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and dot blot data
10-12 weeks
$750.00
Polyclonal package 3 (From peptide design to antiserum)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  2-3mg antigen peptide
l  1-2ml pre-bleed
l  40-60ml post-bleed
l  Project datasheet including ELISA data
12-15 weeks
$840.00
Polyclonal package 4 (From peptide design  to antigen purified antibodies)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Dot blot confirmation
l  2-3mg antigen peptide
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and dot blot data
12-15 weeks
$1015.00
Polyclonal package 5 (From plasmid  to antiserum)
l  Customer provides expression vector
l  Express and purify the antigen protein
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  1mg antigen protein
l  1-2ml pre-bleed
l  40-60ml post-bleed
l  Project datasheet including ELISA data
12-15 weeks
$1060.00
Polyclonal package 6 (From plasmid  to antigen purified antibody)
l  Customer provides expression vector
l  Express and purify the antigen protein
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Dot blot confirmation
l  1mg antigen protein
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and dot blot data
12-15 weeks
$1235.00
Polyclonal package 7 (From cDNA  to antiserum)
l  Customer provides protein information
l  We clone the gene and construct the expression vector for fusion protein
l  Express and purify the antigen protein
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Recombinant vector
l  1mg protein
l  1-2ml pre-bleed
l  40-60ml post-bleed
l  Project datasheet including ELISA data
14-16 weeks
$1675.00
Polyclonal package 8 (From cDNA to antigen purified antibody)
l  Customer provides gene and protein information
l  We clone the gene and construct the expression vector for fusion protein
l  Express and purify the antigen protein
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Dot blot confirmation
l  Recombinant vector
l  1mg protein
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and dot blot data
14-16 weeks
$1850.00
Polyclonal package 9 (Phospho-specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & phosphor- peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unphospho-peptide
l  2-5mg phospho-specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
$1900.00
Polyclonal package 10  (Lys mono-methylated specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & mono-methylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Lys(Me) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
$1900.00
Polyclonal package 11  (Lys dimethylated specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & di-methylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Lys(Me2) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
$1900.00
Polyclonal package 12  (Lys trimethylated specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & tri-methylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Lys(Me3) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
$1900.00
Polyclonal package13  (Arg methylation-specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & methylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Arg(Me) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
$1900.00
Polyclonal package14    (Arg di-methylation (Symmetry)-specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & dimethylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Arg(Me2) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
$1900.00
Polyclonal package15  (Arg di-methylation (Asymmetric)-specific polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  2-3mg peptide/EA (non- & dimethylated peptide)
l  1-2ml pre-bleed
l  2-5mg antibody against unmethylated peptide
l  2-5mg Arg(Me2) specific antibody
l  Project datasheet including ELISA and dot blot data
13-16 weeks
$1900.00
Polyclonal package 16 (Western guaranteed polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  Western blot confirmation
l  2-3mg antigen peptide
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA and Western blot data
15-18 weeks
$2160.00
Polyclonal package17 (Western and IHC guaranteed polyclonal antibody)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize two New Zealand rabbits
l  Titer the immunization by ELISA
l  Post-bleed collection
l  Antigen affinity purification
l  WB and IHC confirmation 
l  2-3mg antigen peptide
l  1-2ml pre-bleed
l  2-5mg affinity purified antibody
l  Project datasheet including ELISA,  Western blot and IHC data
15-18 weeks
$2380.00

 

 

Monoclonal Antibody Service
Package Number
Description
Package Contents
Time
Price
Monoclonal package1 (From protein to ascites purification)
l  Customer provides antigen protein
l  Protein will be checked by SDS-PAGE
l  Immunize four mice (Balb/C)
l  Titer the immunization by ELISA
l  Hybridoma fusion and cloning
l  Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). 
l  IgG isotype determination
l  Ascites are prepared from two hybridoma clones which customer selects.  
l  Antigen affinity purification
l  Dot blot for QC.
l  50ul pre-bleed (1:10dilution)
l  2-5 hybridoma clones
l  2-5mg purified antibody from two hybridoma clones
l  Project datasheet including ELISA,  dot blot and isotype determination data
18-24 weeks
$4410.00
Monoclonal package2 (From peptide design  to ascites purification)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize four mice (Balb/C)
l  Titer the immunization by ELISA
l  Hybridoma fusion and cloning
l  Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). 
l  IgG isotype determination
l  Ascites are prepared from two hybridoma clones which customer selects.  
l  Antigen affinity purification
l  Dot blot for QC.
l  2-3mg peptides
l  50ul pre-bleed (1:10dilution)
l  2-5 hybridoma clones
l  2-5mg purified antibody from two hybridoma clones
l  Project datasheet including ELISA,  dot blot and isotype determination data
20-26 weeks
$4850.00
Monoclonal package3 (From plasmid to ascites purification)
l  Customer provides expression vector
l  Express and purify the antigen protein
l  Immunize four mice (Balb/C)
l  Titer the immunization by ELISA
l  Hybridoma fusion and cloning
l  Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). 
l  IgG isotype determination
l  Ascites are prepared from two hybridoma clones which customer selects.  
l  Antigen affinity purification
l  Dot blot for QC.
l  1mg protein
l  50ul pre-bleed (1:10dilution)
l  2-5 hybridoma clones
l  2-5mg purified antibody from two hybridoma clones
l  Project datasheet including ELISA,  dot blot and isotype determination data
20-26 weeks
$5075.00
Monoclonal package4 (From cDNA to ascites purification)
l  Customer provides gene and protein information
l  We clone the gene and construct the expression vector for fusion protein
l  Express and purify the antigen protein
l  Immunize four mice (Balb/C)
l  Titer the immunization by ELISA
l  Hybridoma fusion and cloning
l  Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). 
l  IgG isotype determination
l  Ascites are prepared from two hybridoma clones which customer selects.  
l  Antigen affinity purification
l  Dot blot for QC.
l  recombinant vector
l  1mg protein
l  50ul pre-bleed (1:10dilution)
l  2-5 hybridoma clones
l  2-5mg purified antibody from two hybridoma clones
l  Project datasheet including ELISA,  dot blot and isotype determination data
22-26 weeks
$5515.00
Monoclonal package5 (Phospho- specific monoclonal antibodies)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize six mice (Balb/C)
l  Titer the immunization by ELISA
l  Hybridoma fusion and cloning
l  Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). 
l  IgG isotype determination
l  Ascites are prepared from two hybridoma clones which customer selects.  
l  Antigen affinity purification
l  Dot blot for QC.
l  2-3mg peptide including non- and phosphor-peptide
l  50ul pre-bleed (1:10dilution)
l  2-5 hybridoma clones specific for phosphor-peptide
l  2 hybridoma clones for non-phospho-peptide
l  2-5mg purified phosphor-specific antibody from two hybridoma clones
l  2-5mg purified non-specific antibody from two hybridoma clones
l  Project datasheet including ELISA,  dot blot and isotype determination data
21-26 weeks
$6175.00
Monoclonal package6 (Western guaranteed monoclonal antibodies)
l  Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
l  Synthesize one peptide (12-22aa, purity>85%)
l  Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l  Immunize six mice (Balb/C)
l  Titer the immunization by ELISA
l  Hybridoma fusion and cloning
l  Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). 
l  IgG isotype determination
l  Ascites are prepared from two hybridoma clones which customer selects.  
l  Antigen affinity purification
l  Western blot for QC.
l  2-3mg peptides
l  50ul pre-bleed (1:10dilution)
l  2-5 hybridoma clones
l  2-5mg purified antibody from two hybridoma clones
l  Project datasheet including ELISA,  Western blot and isotype determination data
20-26 weeks
$7940.00

 

 

 

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